Browsing by Author "Flores, Silvia Karina"
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Item Aprovechamiento de materias primas regionales para el desarrollo de envases comestibles activos y biodegradables(2020-10-06) Kupervaser, María Gabriela; Flores, Silvia Karina; Sosa, Carola AndreaEntre las tecnologías emergentes para la optimización de la preservación de alimentos surge como novedosa alternativa el empleo de películas o recubrimientos comestibles autosoportados que confieren propiedades funcionales específicas. Los trabajos desarrollados en este tema, conducen a incorporar agentes antimicrobianos de fuentes naturales en la formulación de películas, ya que la inclusión de aditivos podría considerarse como una nueva tendencia de envasado activo. Los films o recubrimientos comestibles son láminas delgadas elaboradas con materiales aptos para el consumo y que actúan como una barrera entre el alimento y el ambiente que los rodea, incrementando así la vida útil del producto. Los films obtenidos a partir de polisacáridos, especialmente de almidones, presentan un alto potencial ya que tienen un bajo costo, son transparentes, no confieren olor ni color, poseen una baja permeabilidad al O2 y son biodegradables. Estos sistemas altamente prometedores son capaces de mejorar la calidad, vida útil, seguridad y funcionalidad de los alimentos, y además pueden actuar como carriers de ingredientes activos. En el presente trabajo, se expone la formulación de recubrimientos comestibles a base de almidón de mandioca y adicionados de aceites esenciales (AE), y su posterior evaluación del efecto antimicrobiano frente a la levadura Zygosaccharomyces parabailii, como antecedente de la investigación realizada en el marco de mi tesis de maestría. Sobre la base de los resultados obtenidos en la misma, se propone el actual plan de tesis doctoral que consiste en desarrollar un envase comestible, activo y ecofriendly a base de almidón de mandioca, glicerol y agua, y analizar la viabilidad de la incorporación de pulpas o concentrados de frutas regionales del Nordeste Argentino (mango, guayaba y mamón), estudiando su influencia en las propiedades físicas, mecánicas, de barrera y como fuente natural de polifenoles, antimicrobianos y antioxidantes.Item Determinación de actividad antioxidante y antimicrobiana de extractos de frutas tropicales del nordeste Argentino (NEA)(2022-10-04) Kupervaser, María Gabriela; Traffano Schiffo, María Victoria; Flores, Silvia Karina; Sosa, Carola AndreaLos residuos vegetales de la industrialización han cobrado interés debido a que pueden presentar compuestos antioxidantes y antimicrobianos. En el presente trabajo se estudió el contenido de polifenoles totales (PT) (método Folin-Ciocalteu), la capacidad antioxidante (CA) (método DPPH) y el efecto antimicrobiano frente a las levaduras Zygosaccharomyces parabailii (DMic031766), Zygosaccharomyces bailii (ATCC MYA4549) y a la bacteria Listeria innocua (CIP80.11), de extractos de cáscara (c) y pulpa (p) de frutas tropicales de la región del NEA: mango criollo (MC); mango Keitt (MK); guayaba criolla (GC); papaya criolla (PC) y papaya Formosa (PF). Tanto p y c fueron secadas por convección de aire (12 h, 45 oC), molidas y almacenadas (-18 oC). Las muestras fueron suspendidas en agua destilada para la extracción de PT asistida por baño de ultrasonido (30 min, 40 oC). Posteriormente, los extractos se concentraron por liofilización. La actividad antimicrobiana se analizó mediante ensayos de difusión en agar (halos de inhibición), utilizando una suspensión de microorganismos de ~1.6x106 UFC/mL. El contenido de PT (mg ácido gálico/g extracto) estuvo entre 27.3±0.4 para MCc y GCc, y 1.1±0.3 para MCp. Los cultivares de papaya y la variedad MK, no tuvieron diferencias significativas (p<0.01) entre p y c, siendo el valor medio 5.6±1.1 para PC, 8.7±1.8 para PF y 3.8±1.6 para MK. MCp no difirió de PCc y MK. La GCp (13±1) fue similar a PFp (9.3±1.6). En cuanto a la CA (mg Trolox/g extracto), se obtuvo un rango entre 8.2±0.2 (PCp y PCc) y 2.1±0.1 (GCp). Partes (c y p) de PC, PF (6±1) y MK (3.4±0.3), no presentaron diferencias significativas (p<0.01). MCp fue análogo a PC (c y p) y PFc. GCc (3.7±0.8) fue similar a las partes de MK, MCc y GCp. Los extractos inhibieron en mayor medida a L. innocua: MCp (11.5 mm) y MCc (13 mm); PCp y PCc (12 mm); MKp (11 mm); MKc (18 mm); y PFp (12 mm). Se evidenció una menor respuesta para Z. parabailii: MCp (9.5 mm) y MCc (12 mm) y Z. bailii: MCp (10 mm) y MCc (10 mm); MKp (10 mm); y GCc (12 mm). GC presentó la menor actividad antimicrobiana. Se puede concluir que, en general, la cantidad de PT no se correlacionó con la CA, como es el caso MCc con valor máximo de PT pero una CA reducida (55% por debajo de PC). Contrariamente, MCp mostró el valor más bajo de PT, a la vez que una de las mayores CA. Además, MCp y MCc presentaron efecto inhibitorio para los tres microorganismos ensayados. MKc presentó la máxima inhibición contra L. innnocua, una alta CA pero bajo PT. La cantidad de PT de las variedades criollas del NEA, fue comparable a las comerciales (MK y PF) estando, además, disponibles en grandes cantidades. Por lo tanto, se prevé un agregado de valor de las frutas y un uso promisorio de sus extractos con capacidad antioxidante y/o antimicrobiana para la conservación de alimentos.Item Extracción de compuestos bioactivos en residuos de frutas tropicales : efectos de los métodos de extracción(2021-12-13) Kupervaser, María Gabriela; Traffano Schiffo, María Victoria; Sgroppo, Sonia Cecilia; Sosa, Carola Andrea; Flores, Silvia KarinaEl interés por el consumo de frutas tropicales se encuentra en constante crecimiento debido a sus características sensoriales, nutricionales y funcionales que ejercen un efecto preventivo ante algunas enfermedades fisiológicas. A nivel mundial, las frutas tropicales representan el 3% de los productos agrícolas, con altos volúmenes de exportación (7,8 millones de Tn en 2019) que las posiciona como el tercer grupo más valioso. La industrialización de éstas conlleva a la generación de grandes cantidades de residuos (semillas, cáscaras, hojas), provocando pérdidas económicas y constituyendo un riesgo ambiental. Estudios científicos indican la presencia de cantidades apreciables de compuestos bioactivos en la fracción no comestible, por lo que su reutilización para la obtención de nuevos compuestos resulta promisoria tanto en el aspecto ambiental como económico.Item Gluten free edible films, coatings and toppings(Marina F. de Escalada Pla (Editor); Carolina E. Genevois (Editor), 2023-04-26) Flores, Silvia Karina; García, María Alejandra; Gerschenson, Lía; Kupervaser, María Gabriela; Sosa, Carola Andrea; Traffano Schiffo, María Victoria; Versino, FlorenciaThe gluten intolerances have determined diet changes based on the elimination of ingredients that contain prolamins and glutenin from wheat, rye and barley being replaced, in part, for alternative grains and tubers that do not induce the disease, for instance, rice, corn, sorghum, and millet (Lebwohl and Green 2021). This has led to an important challenge for the food industry due to the need of developing formulation strategies, generally known as “gluten free” (GF) ones, that include the use of suitable additives linked to this dietary modification, while helping to produce safety and organoleptically adequate food products (Zoumpopoulou and Tsakalidou 2019). According to the Food and Drug Administration (U.S.A.), the GF food is defined as the food that does not contain gluten, or its presence should be lower than 20 ppm (McCabe 2010). Bread and sweet baked goods (cakes, biscuits, doughnuts, etc.) are an essential constituent of the human daily diet, representing the most important basic food worldwide (Nils-Gerrit Wunsch 2020; Xu et al. 2020). There is a wide assortment of such products and, a possible classification is the one proposed by Smith et al. (2004) who grouped them as follow: unsweetened (bread, rolls, buns, crumpets, muffins, and bagels), sweet (pancakes, doughnuts, waffles, and cookies) and filled (fruit and meat pies, sausage rolls, pastries, sandwiches, cream cakes, pizza, and quiche) goods. In their formulation, these products include complex carbohydrates (mainly wheat flour), proteins, lipids, vitamins, and minerals (Soukoulis et al. 2014). Another classification proposed is based on the water activity (aw), one of the most important product properties affecting the physical and microbial deterioration of bakery products. Smith and Simpson (1995) classified bakery products as follow: (a) low moisture bakery products (cookies and crackers, aw < 0.6) in which microbiological spoilage is not a problem, (b) intermediate moisture products (chocolate coated, doughnuts, Danish pastries, cream-filled cake, soft cookies, aw 0.6–0.85) where osmophilic yeasts and moulds are the predominant spoilage microorganisms, and (c) high moisture products (bread, pita bread, fruit pies, carrot cake, cheesecake, pizza crust, pizza, aw > 0.85 and generally 0.94–0.99), where almost all bacteria, yeasts, and moulds are capable of growth (Smith et al. 2004). When no preservative additives are added, bread and bakery products are characterized by their limited shelf-life reaching a maximum of 3–5 days at room temperature. After this time, physical, chemical and microbiological changes are produced, resulting in the loss of freshness, texture, taste and microbial spoilage (growth of bacteria, yeast and mould) causing consumer’s rejection (Melini and Melini 2018). Those alterations can cause not only economic losses, but also threaten human health. Therefore, to extend bread and bakery products shelf-life and to assure their quality and safety properties, preservation techniques such as the use of preservatives or adequate packaging materials and the application of innovative processing technologies are proposed (Mitelut et al. 2021; Qian et al. 2021). Over time, one of the most conventional technique applied to extended freshness quality was the use of chemical additives as was previously detailed in Chap. 4. The bakery industry is looking for novel alternatives including the use of antioxidant and antimicrobial compounds obtained from natural sources, new packaging technologies, application of functional coatings, etc. (Klinmalai et al. 2021; Silva et al. 2021; Nallan Chakravartula et al. 2019a). Traditionally, to select a suitable packaging material for bakery products, the most important properties usually sought are gases and water vapour barrier, UV barrier, thermal stability, mechanical resistance (Roy and Rhim 2020). The most used packaging materials to preserve bread are different types of paper, such as waxed paper or the glazed imitation parchment which is strong and has grease resistance. It is usually impregnated on both sides with paraffin wax containing low density polyethylene (LDPE) and other additives (Martins et al. 2021). One alternative is LDPE bags with a strip of adhesive tape at the end to be twisted and sealed. Cakes and pastry products, which are more susceptible to crushing damage, are usually packed in grease-resistant paperboard bags with transparent cellophane windows and wrap, such as cling film, plastic nests or aluminium foil base plates and double plastic film layers. For long shelf-life products (biscuits and other), cellulose films coated with LDPE are generally used (De Pilli 2020; Galić et al. 2009) or other multi-layered films such as aluminium-coated LDPE, oriented polypropylene (OPP) or acrylic-coated OPP films which represent more effective barriers to oxygen and water vapour. In the case of fresh baked stuff immediately consumed, it is commonly packaged in bags made of polyolefin film, such as LDPE or polypropylene bags, normally microperforated to allow moisture to escape and avoid leathery consistency of the crust (Pasqualone 2019). Regarding packaging methods, the application of new technologies such as vacuum packaging, nitrogen flushing, modified atmosphere, functional or active packaging (with antimicrobial activity) reduce the growth of spoilage microorganisms, extending bakery products shelf-life (Qian et al. 2021). It is important to highlight that the plastic derived from fossil hydrocarbons comprise 46% of global plastic waste generation, producing a huge impact to the environment, which often end up in landfill sites or oceans, causing a significant pollution due to the poor infrastructure, the lack of recycling options and to the long periods of time required for their degradation (Tiseo 2021; Geyer et al. 2017). Thus, there is a wide interest in the development of new materials for substituting plastic packaging by using renewable resources to reduce polluting residues. In this framework, biodegradable packaging has emerged as an innovative and promising solution since they decompose after fulfilling their purpose (Chiralt et al. 2020; Tapia-Blácido et al. 2020). New biodegradable materials can be classified in chemically synthesised polymers made from natural or petroleum-based molecules (polylactic acid, polycaprolactone, polyvinyl alcohol, polyglycolic acid, polybutylene succinate, polybutylene adipate-co-terephthalate); directly extracted from biomass (biopolymers such as cellulose, starches, chitosan, alginate, gelatine, collagen, etc.) and biosynthesized via microbial fermentation (polyhydroxyalkanoates, bacterial cellulose) (Zhang et al. 2022; Kamarudin et al. 2022; Birania et al. 2022). These have been used to develop new eco-friendly and active systems that could be applied to protect or improve quality of GF bakery products. In the following sections of this chapter, a special description of biodegradable and edible matrices is performed.Item Non-cereals starch resources(Springer Nature Switzerland AG, 2023-05-26) Dini, Cecilia; Flores, Silvia Karina; Kupervaser, María Gabriela; Sosa, Carola Andrea; Traffano Schiffo, María Victoria; Viña, Sonia ZulmaTo produce bakery goods, the gluten protein is the key factor due to its contribution to water absorption capacity and because it provides extensibility, elasticity and cohesiveness to bread dough. This allows the fermentation gas remains occluded and maintained in the liquid phase during dough development, leading to obtain high-grade breads (Wieser 2007). However, gluten has been identifed as the responsible of celiac disease (Ronda et al. 2009), and the only effective treatment for patients is to follow strictly a gluten-free (GF) diet (Witczak et al. 2016). It has been observed that, if formulation is not properly adjusted, baked products without glu ten could result in lower quality attributes, reduced nutritional characteristics and consumer acceptance (Naqash et al. 2017). Therefore, the development of GF products appropriate for consumers with disorders related to gluten intake was growing in importance (Zhang et al. 2017). Formerly, hydrocolloids and starch were the major ingredients in GF diets (Shi and Bemiller 2002). From last decades, the demand of new food ingredients suitable for GF products is expanding in order to obtain more foods for a wider diet without potentially allergenic proteins. Among those ingredients, alternative starches resources are intensely searching. Starch is one of the most abundant and consumed natural polysaccharide in human diet. It is a biopolymer composed of glucose and it is obtained from plants such as grains, legumes, and tubers (Karmakar et al. 2014). Despite its high abundance, commercially sustainable sources of starch are limited to corn, wheat, cassava, potato and rice. With respect to corn, the global market reached 78 million tons (Mt) in 2020, being mainly produced in the United States, Europe and China (70–80%), whereas wheat is produced (6.3 Mt) mostly in Europe China and India (98%). The cassava starch (CS) production (6.9 Mt) comes from Asian Pacifc region (Thailand, China and Indonesia) and Brazil (75%). Finally, potato starch (PS) which global market attained 3.4 Mt, accounting the highest productions from China, India, United States and Europe (80%); while rice is produced particularly in Asia (Expert Market Research 2020; Murphy 2000). Each region has a more convenient source of starch production mostly determined by climatic and logistic requirements (Semeijn and Buwalda 2018). For food production (noodles, baked goods, etc.), starch is widely used as a gelling, thickening, and/or stabilizing agent (Fonseca et al. 2021; Rożnowski et al. 2014), besides being processed and used as binder, sweetener and as emulsifer (Mahmood et al. 2017; Bello-Pérez et al. 2006). In particular, natural starches with low or without gluten are intensely requested because of their possibility to be used in the formulation of GF bakery products. In this context, corn and potato are the most commonly used starches, together with cassava and rice (Masure et al. 2016), due to their benefcial characteristics, such as neutral taste, soft texture, and high digestibility. They are frequently used in combination with proteins and hydrocolloids to counter their minimal structure-building potential, contributing to the structure, texture and stability of food through their thickening or gelling behaviour and surface properties (Capriles and Arêas 2014; Doublier et al. 2000). Other cereals, like minor or pseudo-cereals, like sorghum, millet, quinoa, amaranths and buckwheat, are being tested as alternative ingredientes tolerated by celiac patients (Comino et al. 2013). In addition, new sources of non-cereal starch are being explored including beans (pea, chickpea), sweet potato and other ethnical tubers, carrots, nuts and some fruits as banana or mango (unripe pulp and kernel) (Witczak et al. 2016; Punia Bangar et al. 2021; Lagunes-Delgado et al. 2022). Till the moment, many reports are found describing general properties of such novel starches but there are not clear applications yet. To better understanding the functionality of starch in food production, some general aspects about structure, functional, nutritional properties and a brief mention to available techniques to modify starches is described in the following sections. A special description and some applications of potato, cassava and others non conventional resources starches is also exposedItem Trends in starch-based edible films and coatings enriched with tropical fruits extracts : a review(2023-05-19) Kupervaser, María Gabriela; Traffano Schiffo, María Victoria; Dellamea, María Luciana; Flores, Silvia Karina; Sosa, Carola AndreaPetroleum-based food packaging materials are non-degradable and considerably affect the environment. In this context, edible films and coatings for food preservation represent a feasible alternative that could potentially reduce conventional non-biodegradable materials. It has been shown the suitability of starch as a non-toxic, widely available, low-cost and adequate film forming biopolymer. In addition, natural compounds contained in tropical fruits are of great interest due to their proven antioxidant and antimicrobial properties, which are beneficial for the health of consumers and for obtaining more stable and safe foods. This review focuses on the new trends and benefits of incorporating tropical fruit extracts into starch-based edible films and coatings formulations as a source of bioactive compounds. The starch and fruit resources, extraction techniques, filmmaking methods, assays for determining functional properties and the potential uses of edible films and coatings in the food industry were stated and described.