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dc.creatorConde Molina, Debora
dc.creatorSanchez Hulmedilla, Betiana
dc.creatorBogao, Guillermina
dc.creatorTubio, Gisela
dc.creatorCorbino, Graciela
dc.date.accessioned2022-12-29T20:36:02Z
dc.date.available2022-12-29T20:36:02Z
dc.date.issued2022-08
dc.identifier.citationSánchez Holmedilla B., Bogao G., Tubio G., Corbino G., Conde Molina D. Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1. Jornadas de Ciencia y Tecnología. Córdoba, Argentina.es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12272/7470
dc.description.abstractThe development of bioprocesses using agro-industrial waste is one of the most interesting challenges in biotechnology today, as it leads to the reuse of waste in an environmentally responsible way and the development of high value-added products. In the San Pedro region, sweet potato production generates considerable volumes of waste, as approximately 40% of the total production is discarded because it does not meet the requirements for commercialisation. In this study, the ability of the strain Cellulosimicrobium sp. CO1A1 to produce xylanases in a medium formulated with sweet potato root residues was evaluated; xylanase being an enzyme of great industrial application. Cellulosimicrobium sp. CO1A1 was grown in minimal saline medium containing 5 % (w/v) sweet potato root extract, at 135 rpm and 25 °C. Xylanase activity was determined using beech xylan (1%) as substrate at 50 ºC, reducing sugars released were determined by the Nelson-Somogyi method. Several assays were performed in order to evaluate xylanase activity, such as the analysis of cell fractions (supernatant -extracellular activity-, cell pellet -cell-associated activity- and cell homogenate -intracellular activity-); culture samples taken at 2, 5 and 7 days of incubation; and media formulated with different sweet potato varieties (Arapey, Beauregard, Covington, Selecta, Morada, Boni). In addition, the xylanase reaction was carried out in two buffer conditions, sodium citrate (50 mM, pH 5.2) and sodium phosphate (50 mM, pH 6.8). The results showed that although Cellulosimicrobium sp. CO1A1 showed growth in the medium formulated with sweet potato root, thus proving that it has the ability to use sweet potato root as a carbon source, it did not show the ability to produce xylanases under the conditions tested, unlike other strains of the genus Cellulosimicrobium reported.es_ES
dc.formatpdfes_ES
dc.language.isoenges_ES
dc.publisherUTN Facultad Regional San Franciscoes_ES
dc.rightsopenAccesses_ES
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.rights.uriAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.subjectFacultad Regional Deltaes_ES
dc.subjectUTNes_ES
dc.subjectSweet potato residueses_ES
dc.subjectXylanaseses_ES
dc.subjectCellulosimicrobium sp.es_ES
dc.titleSweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.es_ES
dc.typeinfo:eu-repo/semantics/conferenceObjectes_ES
dc.rights.holderLos autoreses_ES
dc.description.affiliationFil: Conde Molina, Debora. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.es_ES
dc.description.affiliationFil: Sanchez Hulmedilla, Betiana.Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.es_ES
dc.description.affiliationFil: Bogao, Guillermina. Universidad Tecnológica Nacional. Facultad Regional Delta. Grupo de Biotecnología y Nanotecnología Aplicada; Argentina.es_ES
dc.description.affiliationFil: Tubio, Gisela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.es_ES
dc.description.affiliationFil: Corbino, Graciela. Instituto Nacional de Tecnología Agropecuaria. Estación Experimental Agropecuaria San Pedro; Argentina.es_ES
dc.type.versiondraftes_ES
dc.rights.useAtribución – No Comercial – Sin Obra Derivada (by-nc-nd)es_ES


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