Browsing by Author "Corbino, Graciela"

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    Aprovechamiento de residuos de raíz de batata para la producción de xilanasas bacterianas: un enfoque sustentable.
    (UTN Facultad Regional San Francisco, 2023-09) Conde Molina, Debora; Bogao, Guillermina; Tubio, Gisela; Corbino, Graciela
    La producción del cultivo de batatas genera una cantidad significativa de residuos en la región noroeste de la provincia de Buenos Aires, Argentina. Con el fin desarrollar un bioproceso que emplee residuos de raíz de batata para la producción de xilanasas, enzimas de gran aplicación industrial, se aisló y estudió la cepa BX1. El crecimiento de la bacteria y su actividad de xilanasas se evaluó en cultivos líquidos de MSM (medio salino mínimo)+ batata, MSM + xilano y MSM + cáscara de batata. Los resultados mostraron que la cepa BX1 presentó la capacidad de producir xilanasas en un bioproceso diseñado en 2 etapas, primero un batch con medio MSM + batata (5 %p/v) para generar biomasa, y luego esta biomasa se cosechó y empleó en un batch con medio MSM + xilano (0,5 %p/v) para inducir la producción de xilanasas. Esta actividad enzimática se localizó en la fracción extracelular.
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    Evaluation of xylanse, alpha-amylase and cellulase production from Cellulosimicrobium sp. using a sweet potatoe root residues medium.
    (NUFT, 2021-09) Conde Molina, Débora; Tubio, Gisela; Corbino, Graciela
    Enzymes such as xylanase, alpha-amylase and cellulase are widely used in the food industry, mainly in baking. Most enzyme production processes have focused on fungi as producer of enzymes, however, bacterial producers (have) been less studied. On the other hand, search for low-cost and easily available raw materials that can be used as fermentable substrates is one of the most interesting challenges in biotechnology. In this work, we evaluated a biotechnological process for the valorization of sweet potato root residues as carbon source in order to obtain xylanase, alpha-amylase and cellulose from Cellulosimicrobium sp. The three enzymes evaluated were detected from Cellulosimicrobium sp. CO1A1 when bacteria were harvested at 5 days of incubation. Enzyme activities were not detected in the supernatant culture, however, they were registered in the cell pellet, being 2.1 ± 0.1 U/mL for xylanase, 1.6 ± 0.1 U/mL for alpha-amylase and 0.8 ± 0.1 U/mL for cellulose. We conclude that Cellulosimicrobium sp. CO1A1 is able to produce xylanase, alpha-amylase and cellulose using an alternative low-cost carbon source. Further testing will be needed to study xylanase production from Cellulosimicrobium sp. in order to generate a value-added product from the transformation of a residue product of agricultural activity.
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    Sweet potato root waste: evaluation of a culture medium to produce xylanases from Cellulosimicrobium sp. CO1A1.
    (UTN Facultad Regional San Francisco, 2022-08) Conde Molina, Debora; Sanchez Hulmedilla, Betiana; Bogao, Guillermina; Tubio, Gisela; Corbino, Graciela
    The development of bioprocesses using agro-industrial waste is one of the most interesting challenges in biotechnology today, as it leads to the reuse of waste in an environmentally responsible way and the development of high value-added products. In the San Pedro region, sweet potato production generates considerable volumes of waste, as approximately 40% of the total production is discarded because it does not meet the requirements for commercialisation. In this study, the ability of the strain Cellulosimicrobium sp. CO1A1 to produce xylanases in a medium formulated with sweet potato root residues was evaluated; xylanase being an enzyme of great industrial application. Cellulosimicrobium sp. CO1A1 was grown in minimal saline medium containing 5 % (w/v) sweet potato root extract, at 135 rpm and 25 °C. Xylanase activity was determined using beech xylan (1%) as substrate at 50 ºC, reducing sugars released were determined by the Nelson-Somogyi method. Several assays were performed in order to evaluate xylanase activity, such as the analysis of cell fractions (supernatant -extracellular activity-, cell pellet -cell-associated activity- and cell homogenate -intracellular activity-); culture samples taken at 2, 5 and 7 days of incubation; and media formulated with different sweet potato varieties (Arapey, Beauregard, Covington, Selecta, Morada, Boni). In addition, the xylanase reaction was carried out in two buffer conditions, sodium citrate (50 mM, pH 5.2) and sodium phosphate (50 mM, pH 6.8). The results showed that although Cellulosimicrobium sp. CO1A1 showed growth in the medium formulated with sweet potato root, thus proving that it has the ability to use sweet potato root as a carbon source, it did not show the ability to produce xylanases under the conditions tested, unlike other strains of the genus Cellulosimicrobium reported.
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    Use of sweet potato root residues as carbon source for the growth of xylanase-producing Cellulosimicrobium sp.
    (NUFT, 2021-09) Conde Molina, Débora; Corbino, Graciela
    Application of sweet potato root (SRP) residues as an alternative carbon source to formulate a culture medium for the growth of xylanase-producing microorganisms, is presented in this article. Six commercial varieties of SPR were tested for evaluating the growth of Cellulosimicrobium sp. CO1A strain in shaken flask cultures. Results showed that Cellulosimicrobium sp. CO1A1 was able to grow in a medium formulated with SPR. Biomass estimated by optical density was the most appropriate method for the medium evaluated. Additionally, biomass did not show significant differences (p>0.05) between commercial varieties of SPR, being between 8.12 and 9.26 of OD. All the varieties of RPS tested in this work are equally promising source to be applied in a bioprocess to obtain xylanase by Cellulosimicrobium sp. CO1A1.