Browsing by Author "Verardo, Vito"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Enhancement of extraction parameters for bioactive substances from ocimum basilicum leaves through sonotrode-assisted ultrasonic extraction
    (RICIFA, 2023-11-16) Aloisio, Carolina; Razola-Diaz, María del Carmen; Aznar-Ramos, María José; Longhi, Marcela; Andreatta, Alfonsina E.; Verardo, Vito
    Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutical benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds in plants. However, few works have developed multivariable studies to find the optimal conditions to extract the maximum amount of compounds, especially applied to UAE via sonotrode. The purpose of this work was to perform a multi-variable study employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via sonotrode were ethanol/water ratio, amplitude and time. The analyzed responses were the rosmarinic acid, sum of phenolic acids and sum of phenolic compounds content. Optimal conditions were found to be 50% ethanol/water, 50% amplitude and 5 min. 27 bioactive compounds were identified by HPLC-ESI-TOF-MS, when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.
  • Thumbnail Image
    Item
    Optimization of the extraction conditions of bioactive compounds from Ocimum basilicum leaves using ultrasound-assisted extraction via sonotrode.
    (2023-07-07) Aloisio, Carolina; Razola-Diaz, María del Carmen; Aznar-Ramos, María José; Longhi, Marcela; Andreatta, Alfonsina E.; Verardo, Vito
    Sweet basil (Ocimum basilicum) leaves are rich in bioactive compounds that present therapeutic benefits for human health. Ultrasonic-assisted extraction (UAE) is frequently used to obtain phenolic compounds from plants/herbal sources. However, few works have developed multi-variable studies to find the optimal conditions to extract the maximum amount of compounds, especially when applied to UAE via a sonotrode. The purpose of this work was to perform a multi-variable study by employing a Box–Behnken design to collect the highest active compound content from Ocimum basilicum leaves. The efficacy of the design was endorsed by ANOVA. The studied parameters for UAE via a sonotrode were the ethanol/water ratio, amplitude, and time. The analyzed responses were the rosmarinic acid, the sum of phenolic acids, and the sum of phenolic compounds content. The optimal conditions were found to be 50% ethanol/water, 50% amplitude, and 5 min. Twenty bioactive compounds were identified by HPLC-ESI-TOF-MS when the extract was collected by applying the optimal conditions. Ocimum basilicum may be appreciated as a valuable source of important bioactive substances for pharmaceutical use.