Browsing by Author "da Cruz Cabral, Lucía Mariana"

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    Alternaria in malting barley: characterization and distribution in relation with climatic conditions and barley cultivars
    (Elsevier, 2021-08-24) Castañares, Eliana; da Cruz Cabral, Lucía Mariana; Dinolfo, María I.; Andersen, Birgitte; Stenglein, Sebastián A.; Patriarca, Andrea
    Alternaria is one of the main fungal genera affecting the quality of barley grains. In this study, a polyphasic approach was carried out to characterise the Alternaria population infecting different cultivars of barley grains from the major producing regions of Argentina in the 2014 and 2015 seasons. Its relationship with Fusarium and correlations between predominant species, barley cultivars, and climatic conditions in the growing regions were evaluated. Alternaria incidence exceeded that of Fusarium in all the barley samples and was higher in the drier season (21% in 2014 and 42% in 2015 vs. 6% and 4%, respectively). The main Alternaria species-groups identified were present in both growing seasons in similar frequencies (A. tenuissima sp.-grp., 83.4% in 2014 and 81.7% in 2015; A. infectoria sp.-grp., 11.7% in 2014 and 11.3% in 2015). The dominant Alternaria species-group isolated and identified based on morphological characteristics, DNA sequencing, and metabolite profile was A. tenuissima (72.9%), followed by A. infectoria (14.6%). An association between their frequency and field temperature was observed; A. tenuissima sp.-grp. was more frequent in northern localities, where higher temperatures were registered, while the opposite was observed for A. infectoria sp.-grp. A smaller percentage of A. arborescens sp.-grp. (5%), A. alternata sp.-grp. (3.9%) and A. vaccinii (1.4%) were also identified. Both secondary metabolite profiles and phylogenetic analysis were useful to distinguish isolates from Alternaria section Alternaria and section Infectoriae. Regarding metabolite profiles, alternariol was the most frequent compound produced by isolates of the section Alternaria. Infectopyrones and novae-zelandins were produced by most of the isolates from section Infectoriae. The barley cultivars analysed in this study did not show a particular susceptibility regarding the Alternaria population composition, except for Andreia, which presented the highest frequency of contamination with A. tenuissima sp.-grp. The rest of the cultivars, when grown in different regions, showed different proportion of the Alternaria sp.-grps., suggesting that other factors were determinant in their distribution. The results obtained in the present study will be a valuable tool for health authorities to assess the need for regulations on Alternaria mycotoxins, given the high incidence of Alternaria spp. in barley and the diversity of metabolites that might contaminate the grains.
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    Análisis de comunidades microbianas de gorgojos de la tribu Naupactini (Coleoptera) : ¿existe relación entre la microbiota y el modo de reproducción?
    (Sociedad Entomológica Argentina, 2022) da Cruz Cabral, Lucía Mariana; Lanteri, Analía; Confalonieri, Viviana; Rodriguero, Marcela S.
    Resúmen del XI Congreso Argentino y XII Congreso Latinoamericano de Entomología. El objetivo de este trabajo fue describir y comparar las microbiotas de dos especies bisexuales y dos partenogenéticas a fin de proponer interacciones bacterianas que impidan la inducción de partenogénesis en las primeras.
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    Los colores y la acidez de las sustancias que nos rodea; ¿experimentamos?
    (edUTecNe, 2022) da Cruz Cabral, Lucía Mariana; Garralda, Ximena; Primost, Mónica A.
    La propuesta consistió en una experiencia de laboratorio, coordinada por investigadoras y acompañadas por la/el docente de la asignatura Química de 5to año de distintas escuelas secundarias de la ciudad, en la cual los y las estudiantes evaluaron la acidez de diversas sustancias que los rodean, una temática que se aborda en ambos niveles educativos (medio y universitario). La misma se llevó a cabo en formato híbrido: los y las estudiantes de nivel medio pudieron realizar las actividades propuestas en sus aulas o laboratorios, pero guiadas por investigadoras de la facultad. Para ello, se diseñó un “kit de experimentación”, que consistió en una caja que contenía una guía con el paso a paso de la actividad y preguntas disparadoras para experimentar en el aula, junto a todos los materiales necesarios para desarrollarla y un video tutorial filmado en los laboratorios de la facultad con las observaciones de cada etapa del experimento. Entre los materiales entregados, se incluyó el indicador ácido-base (extracto de repollo colorado) y distintas sustancias utilizadas en la vida cotidiana para ser evaluados. Con esta propuesta se buscó consolidar y facilitar el desarrollo de las experiencias prácticas con una resignificación de estas a partir del uso del laboratorio y construcción de argumentos en Química. La premisa fue promover la idea de que lo experimental adquiera mayor protagonismo en la educación de estudiantes, fomentando, además, el trabajo en equipo y la articulación entre los/as profesores del área de nivel secundario y universitario.
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    Effect of Debaryomyces hansenii and the antifungal PgAFP protein on Alternaria spp. growth, toxin production, and RHO1 gene expression in a tomato-based medium
    (Elsevier, 2021-01-20) da Cruz Cabral, Lucía Mariana; Rodríguez, Alicia; Andrade, María J.; Patriarca, Andrea; Delgado, Josué
    Tomato fruit is susceptible to Alternaria spp. spoilage, which poses a health risk due to their mycotoxin production. Biopreservation relies on the use of whole microorganisms or their metabolites to manage spoilage microorganisms including filamentous fungi. However, the use of treatments at fungistatic level might activate intracellular pathways, which can cause an increment in mycotoxin accumulation. The objective of this work was to evaluate the effect of two strains of Debaryomyces hansenii and the antifungal protein PgAFP at 10 and 40 μg/mL. Both growth and production of two of the most common mycotoxins (tenuazonic acid and alternariol monomethyl ether) by Alternaria tenuissima sp.-grp. and Alternaria arborescens sp.-grp. on a tomato-based matrix, were analysed at 12 °C. Additionally, the impact of these biocontrol agents on the stress-related RHO1 gene expression was assessed. All treatments reduced mycotoxin accumulation (from 27 to 92% of inhibition). Their mode of action against Alternaria spp. in tomato seems unrelated to damages to fungal cell wall integrity at the genomic level. Therefore, the two D. hansenii strains (CECT 10352 and CECT 10353) and the antifungal protein PgAFP at 10 μg/mL are suggested as biocontrol strategies in tomato fruit at postharvest stage.
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    Estudio de indicadores microbiológicos en moluscos bivalvos patagónicos de interés comercial
    (Universitaria de la Patagonia EDUPA, 2022) da Cruz Cabral, Lucía Mariana; Primost, Mónica A.; Castaños, Cecilia
    El conocimiento de bacterias que colonizan alimentos es importante tanto desde la inocuidad alimentaria, como para optimizar las condiciones de almacenamiento y distribución. Los productos pesqueros son altamente susceptibles al deterioro microbiológico, el cual depende de factores intrínsecos de los productos, y de la composición de la microbiota inicial, entre otros.
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    Relevamiento de plantas pesqueras en la provincia del Chubut
    (Universitaria de la Patagonia EDUPA, 2022) Castaños, Cecilia; Gurisich, Soledad; Schulze, María Soledad; Góngora, María Eva; Corvalán, Soraya; Fernández, Cristina Andrea; da Cruz Cabral, Lucía Mariana
    El Sistema Pesquero del Mar Argentino involucra un sistema socio-ecológico-productivo complejo donde las relaciones sociales de producción y el mercado de trabajo constituyen un proceso dinámico que se modifica a lo largo del tiempo.
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    Wolbachia-density variation in weevils of the tribe Naupactini (Coleoptera, Curculionidae)
    (2024-01-20) da Cruz Cabral, Lucía Mariana; Fernandez Goya, Lucía; Piccinali, Romina V.; Lanteri, Analía; Confalonieri, Viviana A.; Rodriguero, Marcela S.
    The intracellular bacteria Wolbachia pipientis infects arthropods and filarial nematodes and is able to manipulate host reproduction. It has been reported an association between parthenogenesis and Wolbachia infection in weevils from the tribe Naupactini. A curing experiment suggested that a threshold density of Wolbachia is required for parthenogenetic reproduction to occur. The aim of this study was to analyze Wolbachia infection status and density in two sexually reproducing species from the tribe Naupactini, Naupactus xanthographus and Naupactus dissimulator. Wolbachia infection was detected in individuals from both species in several geographic locations, not being fixed. Quantification through real time PCR confirmed that Wolbachia loads in sexual species were significantly lower than in parthenogenetic ones; these results support the hypothesis of a threshold level for parthenogenetic reproduction to occur in Naupactini weevils. Strain typing showed that both sexual species carry wNau1, the most frequent strain in parthenogenetic Naupactini weevils. In addition, the presence of the WO phage, which might be an important factor regulating infection density in some hosts, was detected in this strain. Finally, Wolbachia wNau1 was located throughout the whole insect body, which is in agreement with the idea of a recent acquisition by horizontal transfer of wNau1 across the tribe Naupactini.