Grupo de Procesos Biotecnológicos

Permanent URI for this collectionhttp://48.217.138.120/handle/20.500.12272/1233

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Author: search.filters.author.Liporace, FrancoSubject: search.filters.subject.FRD

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    Development of bioremediation strategies based on the improvement of biomass production from isolated strains in hydrocarbon contaminated soils and their application in bioremediation technologies.
    (2019-07-07) Conde Molina, Débora; Liporace, Franco; Quevedo, Carla
    Contaminated sites with petroleum compounds are frequently observed, requiring the development of innovative technologies for its remediation. The problem is caused due to the widespread usage of petroleum-based products. Their discharge and accidental spillage in the environment prove to be hazardous both to the surroundings and life forms. Bioremediation is an efficient strategy for cleaning up sites contaminated with organic pollutants. It is a non-invasive and cost-effective technique that relies on natural decontamination using microbes of isolated strains from contaminated areas for the clean-up of these petroleum hydrocarbons. The Zárate-Campana industrial center, located in Buenos Aires, represents one of the most important petrochemical areas in Argentina, with several companies carrying out petrochemical activities. In this study, we have investigated the ability of microorganisms to degrade these hydrocarbons. Samples were collected in the surroundings of the Campana area and screened for hydrocarbon degrading bacteria. 4 of the 13 strains previously isolated from contaminated sites were screened and identified as Pseudomonas sp, Cellulosimicrobium sp and Ochrobactrum sp. A new approach using MT1A3, belonging to Pseudomonas genus in petroleum biodegradation from the use of different carbon and nitrogen sources, was proposed to provide maximum biomass production and was evaluated for its degradation characteristics. MT1A3 grew in all carbon sources tested and was able to grow in a hydrocarbon mixture obtaining 1.79 g/L of biomass production at 25 ºC after 7 days. When comparing the use of different low-cost agro-industrial co-products as an alternative carbon source, the biomass production was significantly higher in crude peanut oil in comparison to all other substrates (p < 0.05), thus resulting in a biomass of 7.29 g/L. The most efficient nitrogen source for obtaining biomass from MT1A3 was NaNO3. Based on these results, the effectiveness was evaluated by monitoring total hydrocarbons (THs) and n-alkanes degradation as well as changes in bacterial population of natural attenuation, biostimulation and bioaugmentation treatments in microcosm design over a 120-day period. The best treatment, which involved bioaugmentation (MT1A3) and biostimulation strategies, showed a degradation of 40.05 % of total hydrocarbons with respect to the natural attenuation treatment used as control. The highest concentration of THAB and HDB was recorded, reaching a value of 2,17x1010 CFU and 8,91x106 UFC respectively.
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    Estudio de cepa autóctona aislada de suelos contaminados con hidrocarburos para su utilización en tecnologías de biorremediación
    (Asociación Argentina de Microbiología, 2018) Conde Molina, Débora; Liporace, Franco; Quevedo, Carla
    Se aislaron cepas degradadoras de hidrocarburos de zonas contaminadas en Campana (Bs.As). El aislamiento se llevó a cabo usando cultivos de enriquecimiento: medio salino mínimo (MSM) suplementado con una mezcla de hidrocarburos comerciales (HC) como única fuente de carbono. Paralelo a esto, se estudió la capacidad de biosíntesis de surfactantes de estas cepas a través de la medición de la tensión superficial (ST). La cepa AgHC, perteneciente a género Pseudomonas, fue seleccionada para realizar los ensayos de optimización utilizando diferentes fuentes de carbono. Los cultivos fueron incubados durante 48h en MSM con glucosa o glicerol como sustratos. La biomasa obtenida en esta etapa se transfirió a MSM fresco con HC durante 72h. AgHC fue capaz de crecer utilizando todas las fuentes de carbono ensayadas. En cuanto a la medición ST, mostró ser menor cuando se usaron Glucosa y Glicerol. No se observó un descenso significativo cuando se utilizó HC