Facultad Regional Resistencia

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Author: search.filters.author.Echeverría, Macarena CelesteSubject: search.filters.subject.actinobacteria

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    Efficient furfural removal using actinobacteria from polluted sites : microbial consortium design and application
    (Wiley‐VCH GmbH, 2025-07-01) Echeverría, Macarena Celeste; Costa‐Gutierrez, Stefanie Bernardette; Raimondo, Enzo Emanuel; Polti, Marta Alejandra; Benimeli, Claudia Susana
    Las aguas residuales de una planta de furfural en Argentina contienen 791 mg L⁻¹ de furfural, lo que representa riesgos de toxicidad si no se tratan. Este estudio tuvo como objetivo aislar actinobacterias de sitios contaminados con furfural, seleccionar cepas tolerantes y evaluar su eficiencia de remoción de manera individual y en consorcio. Se aislaron seis microorganismos con características macroscópicas correspondientes al filo Actinomycetota. Estos microorganismos y Streptomyces sp. A5, A12 y M7, aislados de ambientes contaminados con pesticidas y metales pesados, mostraron tolerancia al furfural a 800 mg L⁻¹. El aislado L9 (identificado como Nocardiopsis sp. L9) y Streptomyces sp. A12 y M7 fueron seleccionados porque resultaron los más eficientes en cuanto a su capacidad de crecimiento y remoción de furfural en MM suplementado con furfural a 400 mg L⁻¹. El consorcio formulado con las tres actinobacterias (L9‐A12‐M7) exhibió un crecimiento significativamente mayor (123%) y una eficiencia de remoción de furfural (58%) en comparación con los cultivos individuales, cuando se expuso a una concentración de contaminante similar a la del efluente real (800 mg L⁻¹). Las pruebas de ecotoxicidad utilizando semillas de Raphanus sativus mostraron que los efectos tóxicos causados por el furfural fueron revertidos por el tratamiento, confirmando la efectividad del proceso de biorremediación. Estos resultados sugieren que el consorcio de actinobacterias es una herramienta prometedora de biorremediación para el tratamiento de efluentes industriales contaminados con furfural.
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    Furfural removal from a polluted effluent by using a fluidized bed reactor
    (2023-10-02) Echeverría, Macarena Celeste; Cuadra, Pablo Nicolás; Sandoval, Evangelina; Benimeli, Claudia Susana
    Furfural is a heterocyclic aromatic aldehyde and wastewaters derived from its production can contain around 800 mg l-1, which can cause toxic effects on living systems if released into the environment without proper treatment. In the present work, the furfural removal from a simulated effluent by a fluidized bed bioreactor filled with an actinobacteria biofilm on vegetable sponge (Luffa aegyptiaca) support was studied. For this, a suspension of a mixed culture of Nocardiopsis sp. L9, Streptomyces sp. A12 and M7, in TSB medium (D.O540nm=1) was prepared. The luffa support was cut in cubes of approximately 0.1 g, which were washed and sterilized. The bacterial biofilm production on luffa cubes was carried out in 250 ml Erlenmeyer flasks, which contained 0.5 g of the support and 60 ml of the bacterial suspension. After 96 h of incubation at 30 °C and 100 rpm, the colonized sponge cubes were introduced into the reactor for the bioremediation treatment. A laboratory-scale fluidized bed reactor was used, which had an inlet for the effluent to be treated in the lower side and an outlet for the treated effluent in the upper part. The furfural residual concentration in the treated effluent was evaluated by HPLC, every 24 h for 4 days. Ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). Bacterial colonization on vegetal sponge was also evaluated by scanning electron microscopy, before and after treatment. The analysis by HPLC showed a complete depletion of furfural in the effluent after 24 h of treatment. Microphotographs by scanning electron microscopy showed an increase in the presence of possible polymeric substances in luffa cubes at the end of treatment regarding to the initial time, as result of biofilm production by the actinobacterial consortium. The ecotoxicity tests with radish seeds showed significant increases (p<0.05) in the vegetable biomarkers of seedlings obtained in the treated effluent, indicating that the toxic effects caused by furfural were reversed, confirming the effectiveness of the bioremediation process.