A new approach to fishery waste revalorization to enhance Nile tilapia (Oreochromis niloticus) digestion process.
Rodriguez, Yamila Eliana
Harán, Nora Selma
Mallo, Juan Carlos
Fernández Giménez, Analía Verónica
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The main goal of this research was to analyse in vitro compatibility of Nile tilapia (Oreochromis niloticus) digestive proteinases and enzymes recovered from species comprising fishery waste as Pleoticus muelleri, Artemesia longinaris and Patagonotothen ramsayi. It evaluated the use of exogenous enzymes as feed supplements to increase digestion efficiency in tilapia fingerlings and juveniles (3.5 ± 0.11 g and 11.6 ± 1.5 g, respectively). We successfully have obtained proteinases from fishery waste as source of exogenous enzymes. P. muelleri and A. longinaris enzymes had more activity of acid and alkaline proteinases than P. ramsayi. SDS-PAGE gels demonstrated that Nile tilapia digestive proteinases keep their activity when combined with each exogenous proteinase. Exogenous enzymes varied in their ability to enhance hydrolysis of different feed ingredients. P. muelleri by-products are the best candidates to be employed as feed supplements for tilapia juveniles. Enzymes from this by-product did not affect the activity and integrity of fish digestive enzymes, improved the hydrolysis of different protein sources (fish meal, squid meal, shrimp meal and soybean meal), and maintained its activity after being exposed to high temperatures and acid pHs. Our findings are applicable to other places where O. niloticus is raised utilizing local fishery waste, andalso to different cultured species.
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