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Author: search.filters.author.Conde Molina, DeboraSubject: search.filters.subject.Bioaugmentation

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    Bioremediation of an industrial soil contaminated by hydrocarbons in microcosm system, involving bioprocesses utilizing co-products and agro-industrial wastes
    (2023-09-29) Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
    The present study describes practical implication of bioaugmentation and biostimulation processes for bioremediation of an industrial soil chronically contaminated by hydrocarbons. For this purpose, biomass production of six autochthonous hydrocarbon-degrading bacteria were evaluated as inoculum of bioaugmentation strategy, by testing carbon and nitrogen sources included co-products and agro-industrial waste as sustainable and low-cost components of the growth medium. Otherwise, biostimulation was approached by the addition of optimized concentration of nitrogen and phosphorus. Microcosm assays showed that total hydrocarbons (TH) were significantly removed from chronically contaminated soil undergoing bioremediation treatment. Systems Mix (bioaugmentation); N,P (biostimulation) and Mix + N,P (bioaugmentation and biostimulation) reached higher TH removal, being 89.85%, 91.00%, 93.04%, respectively, comparing to 77.83% of system C (natural attenuation) at 90 days. The increased heterotrophic aerobic bacteria and hydrocarbon degrading bacteria counts were according to TH biodegrading process during the experiments. Our results showed that biostimulation with nutrients represent a valuable alternative tool to treat a chronically hydrocarbon-contaminated industrial soil, while bioaugmentation with a consortium of hydrocarbon degrading bacteria would be justified when the soil has a low amount of endogenous degrading microorganisms. Furthermore, the production of inoculum for application in bioaugmentation using low-cost substrates, such as industrial waste, would lead to the development of an environmentally friendly and attractive process in terms of cost–benefit.
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    Site-specific bioremediation by bioaugmentation and biostimulation approaches on a chronically hydrocarbon-contaminated industrial soil.
    (2023-09) Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
    The present study describes practical implication of bioaugmentation and biostimulation processes for bioremediation of an industrial soil chronically contaminated by hydrocarbons. For this purpose, biomass production of six autochthonous hydrocarbon-degrading bacteria were evaluated as inoculum of bioaugmentation strategy, by testing carbon and nitrogen sources included co-products and agro-industrial waste as sustainable and low-cost components of the growth medium. Otherwise, biostimulation was approached by the addition of optimized concentration of nitrogen and phosphorus. Microcosm assays showed that total hydrocarbons (TH) were signifcantly removed from chronically contaminated soil undergoing bioremediation treatment. Systems Mix (bioaugmentation); N,P (biostimulation) and Mix+N,P (bioaugmentation and biostimulation) reached higher TH removal, being 89.85%, 91.00%, 93.04%, respectively, comparing to 77.83% of system C (natural attenuation) at 90 days. The increased heterotrophic aerobic bacteria and hydrocarbon degrading bacteria counts were according to TH biodegrading process during the experiments. Our results showed that biostimulation with nutrients represent a valuable alternative tool to treat a chronically hydrocarbon-contaminated industrial soil, while bioaugmentation with a consortium of hydrocarbon degrading bacteria would be justifed when the soil has a low amount of endogenous degrading microorganisms. Furthermore, the production of inoculum for application in bioaugmentation using low-cost substrates, such as industrial waste, would lead to the development of an environmentally friendly and attractive process in terms of cost–beneft.
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    Removal of vegetable oils from contaminated coastal soil by bioaugmentation and biostimulation
    (Congreso Latinoamericano de Ecología Microbiana, 2023-08) Conde Molina, Debora; Corpus, Athina; Piperata, Gabriela
    The environment is negatively impacted by occasional discharges from industrial activity. When these events contain insoluble compounds such as fats and oils, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Zárate-Campana, Buenos Aires, Argentina, where a lagoon was contaminated by vegetable oil residues. The aim of this work is to study bioremediation strategies in order to propose solutions for the remediation of the lagoon. For this purpose, different site-specific treatments were evaluated through microcosms systems using contaminated coastal soil. These treatments were: a control as natural attenuation; a bioaugmentation treatment with autochthonous vegetable oil degrading bacteria (5x1010 CFU/g); two biostimulation treatments with nitrogen (NaNO3, 1 g/Kg) and phosphorus (Na2HPO4, 0.2 g/Kg), and with spent mushroom substrate (10 %w/w). The microcosms carried out with 200 g of soil were incubated at 22 °C for 60 days. Samples were taken every 20 days in order to determine moisture, pH, biological activity by counting total aerobic heterotrophic bacteria and oil degrading bacteria, and total oil concentration through FTIR. The treatments showed oil removal above 43 %, being the most efficient the biostimulation with nitrogen and phosphorus, which reached 58 % degradation after 60 days. Furthermore, the evolution of oil degradation correlated with the increase in microbiological activity in all systems. We concluded that biostimulation with nitrogen and phosphorus was the most appropriate strategy to apply for lagoon remediation.
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    Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
    (Encuentro Internacional de Ciencias de la Tierra, 2022-11) Conde Molina, Debora; Sanchez Hulmedilla, Celene; Silva, Fausto; Piperata, Gabriela
    The environment is negatively impacted by occasional discharges from industrial activity. When these discharges contain insoluble compounds such as fats, oils and grease, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Campana, Buenos Aires, where a vegetable oil residues treatment industry generated a significant uncontrolled discharge of waste into Lagoon 3 of the private nature reserve El Morejón. In view of this, there is a need to address bioremediation strategies to clean up the area. The aim of this work is to study growth conditions of vegetable oil degrading bacterial consortia, previously isolated from lagoon 3, with a view to applying it as an inoculum in bioaugmentation strategy. For this purpose, bacteria were tested in flask containing liquid culture media at different conditions, such as: medium formulated with 2-5 % v/v vegetable oil -as only carbon source-, shaking at 135-220 rpm, medium formulated with alternative carbon source of sweet potato waste (5% w/v). The best condition for the growth of bacteria was a culture medium formulated with 5 % v/v vegetable oil, incubated at 135 rpm for days, reaching 9 g/L of biomass. Moreover, this condition maintains the selection pressure so that bacteria preserve the ability to degrade vegetable oils. The significant biomass obtained positions these autochthonous bacteria with great potential to be applied as bioaugmentation in site-specific bioremediation strategy for the remediation of Lagoon 3.