FRD - Investigación - Ciencia y Tecnología

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Author: search.filters.author.Piperata, GabrielaSubject: search.filters.subject.Bioremediation

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    Vegetable oil contaminated sites: bioremediation treatments.
    (2023-07) Conde Molina, Debora; Corpus, Athina; Piperata, Gabriela
    The environment is negatively impacted by occasional discharges from industrial activity. When these events contain insoluble compounds such as fats and oils, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Buenos Aires, Argentina, where a lagoon was contaminated by vegetable oil residues. The aim of this work is to study bioremediation strategies in order to propose solutions for the remediation of the lagoon. For this way, autochthonous vegetable oil degrading bacteria were isolated from Lagoon 3, and the conditions to produce bacterial biomass were evaluated. Then, through microcosms systems using contaminated coastal soil, different site-specific treatments were tested: a control as natural attenuation; a bioaugmentation treatment with autochthonous vegetable oil degrading bacteria; two biostimulation treatments with nitrogen and phosphorus, and with spent mushroom substrate. Although both bioaugmentation and biostimulation showed promising results, biostimulation with N, P was the most effective for site-specific bioremediation of Lagoon 3, achieving 67% of oil vegetable reduction at 60 days.
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    Removal of vegetable oils from contaminated coastal soil by bioaugmentation and biostimulation
    (Congreso Latinoamericano de Ecología Microbiana, 2023-08) Conde Molina, Debora; Corpus, Athina; Piperata, Gabriela
    The environment is negatively impacted by occasional discharges from industrial activity. When these events contain insoluble compounds such as fats and oils, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Zárate-Campana, Buenos Aires, Argentina, where a lagoon was contaminated by vegetable oil residues. The aim of this work is to study bioremediation strategies in order to propose solutions for the remediation of the lagoon. For this purpose, different site-specific treatments were evaluated through microcosms systems using contaminated coastal soil. These treatments were: a control as natural attenuation; a bioaugmentation treatment with autochthonous vegetable oil degrading bacteria (5x1010 CFU/g); two biostimulation treatments with nitrogen (NaNO3, 1 g/Kg) and phosphorus (Na2HPO4, 0.2 g/Kg), and with spent mushroom substrate (10 %w/w). The microcosms carried out with 200 g of soil were incubated at 22 °C for 60 days. Samples were taken every 20 days in order to determine moisture, pH, biological activity by counting total aerobic heterotrophic bacteria and oil degrading bacteria, and total oil concentration through FTIR. The treatments showed oil removal above 43 %, being the most efficient the biostimulation with nitrogen and phosphorus, which reached 58 % degradation after 60 days. Furthermore, the evolution of oil degradation correlated with the increase in microbiological activity in all systems. We concluded that biostimulation with nitrogen and phosphorus was the most appropriate strategy to apply for lagoon remediation.
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    Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
    (Encuentro Internacional de Ciencias de la Tierra, 2022-11) Conde Molina, Debora; Sanchez Hulmedilla, Celene; Silva, Fausto; Piperata, Gabriela
    The environment is negatively impacted by occasional discharges from industrial activity. When these discharges contain insoluble compounds such as fats, oils and grease, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Campana, Buenos Aires, where a vegetable oil residues treatment industry generated a significant uncontrolled discharge of waste into Lagoon 3 of the private nature reserve El Morejón. In view of this, there is a need to address bioremediation strategies to clean up the area. The aim of this work is to study growth conditions of vegetable oil degrading bacterial consortia, previously isolated from lagoon 3, with a view to applying it as an inoculum in bioaugmentation strategy. For this purpose, bacteria were tested in flask containing liquid culture media at different conditions, such as: medium formulated with 2-5 % v/v vegetable oil -as only carbon source-, shaking at 135-220 rpm, medium formulated with alternative carbon source of sweet potato waste (5% w/v). The best condition for the growth of bacteria was a culture medium formulated with 5 % v/v vegetable oil, incubated at 135 rpm for days, reaching 9 g/L of biomass. Moreover, this condition maintains the selection pressure so that bacteria preserve the ability to degrade vegetable oils. The significant biomass obtained positions these autochthonous bacteria with great potential to be applied as bioaugmentation in site-specific bioremediation strategy for the remediation of Lagoon 3.