Facultad Regional Delta

Permanent URI for this communityhttp://48.217.138.120/handle/20.500.12272/95

Browse

Author: search.filters.author.Conde Molina, DeboraStart date: 2023

Search Results

Now showing 1 - 7 of 7
  • Thumbnail Image
    Item
    Enhancing biodegradation of vegetable oil-contaminated soil with soybean texturized waste, spent mushroom substrate, and stabilized poultry litter in microcosm systems.
    (2024-06) Conde Molina, Debora; Di Gregorio, Vanina
    Industrial activities contribute to environmental pollution, particularly through unregulated effluent discharges, causing adverse effects on ecosystems. Vegetable oils, as insoluble substances, exacerbate this pollution, forming impermeable films and affecting the oxygen transfer, leading to serious habitat disruption. Organic wastes, such as soybean texturized waste, spent mushroom substrate, and stabilized poultry litter, were assessed for their efficacy in enhancing the degradation of vegetable oil in contaminated soil. For this purpose, contaminated soil was amended with each of the wastes (10% w/w) using microcosm systems, which were monitored physico-chemically, microbiologically and toxicologically. Results indicate that the wastes promoted significant oil degradation, achieving 83.1, 90.7, and 86.2% removal for soybean texturized waste, spent mushroom substrate, and stabilized poultry litter, respectively, within a 90-day period. Additionally, they positively influenced soil microbial activity, as evidenced by increased levels of culturable microorganisms and hydrolytic microbial activity. While bioassays indicated no phytotoxicity in most cases, soybean texturized waste exhibited inhibitory effects on seed germination and root elongation of Lactuca sativa. This study significantly enhances our comprehension of remediation techniques for sites tainted with vegetable oils, highlighting the critical role of organic waste as eco-friendly agents in soil restoration. Emphasizing the practical implications of these findings is imperative to underscore the relevance and urgency of addressing vegetable oil contamination in soil. Moving forward, tailored strategies considering both contaminant characteristics and soil ecosystem traits are vital for ensuring effective and sustainable soil remediation.
  • Thumbnail Image
    Item
    Bioremediation of an industrial soil contaminated by hydrocarbons in microcosm system, involving bioprocesses utilizing co-products and agro-industrial wastes
    (2023-09-29) Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
    The present study describes practical implication of bioaugmentation and biostimulation processes for bioremediation of an industrial soil chronically contaminated by hydrocarbons. For this purpose, biomass production of six autochthonous hydrocarbon-degrading bacteria were evaluated as inoculum of bioaugmentation strategy, by testing carbon and nitrogen sources included co-products and agro-industrial waste as sustainable and low-cost components of the growth medium. Otherwise, biostimulation was approached by the addition of optimized concentration of nitrogen and phosphorus. Microcosm assays showed that total hydrocarbons (TH) were significantly removed from chronically contaminated soil undergoing bioremediation treatment. Systems Mix (bioaugmentation); N,P (biostimulation) and Mix + N,P (bioaugmentation and biostimulation) reached higher TH removal, being 89.85%, 91.00%, 93.04%, respectively, comparing to 77.83% of system C (natural attenuation) at 90 days. The increased heterotrophic aerobic bacteria and hydrocarbon degrading bacteria counts were according to TH biodegrading process during the experiments. Our results showed that biostimulation with nutrients represent a valuable alternative tool to treat a chronically hydrocarbon-contaminated industrial soil, while bioaugmentation with a consortium of hydrocarbon degrading bacteria would be justified when the soil has a low amount of endogenous degrading microorganisms. Furthermore, the production of inoculum for application in bioaugmentation using low-cost substrates, such as industrial waste, would lead to the development of an environmentally friendly and attractive process in terms of cost–benefit.
  • Thumbnail Image
    Item
    Vegetable oil contaminated sites: bioremediation treatments.
    (2023-07) Conde Molina, Debora; Corpus, Athina; Piperata, Gabriela
    The environment is negatively impacted by occasional discharges from industrial activity. When these events contain insoluble compounds such as fats and oils, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Buenos Aires, Argentina, where a lagoon was contaminated by vegetable oil residues. The aim of this work is to study bioremediation strategies in order to propose solutions for the remediation of the lagoon. For this way, autochthonous vegetable oil degrading bacteria were isolated from Lagoon 3, and the conditions to produce bacterial biomass were evaluated. Then, through microcosms systems using contaminated coastal soil, different site-specific treatments were tested: a control as natural attenuation; a bioaugmentation treatment with autochthonous vegetable oil degrading bacteria; two biostimulation treatments with nitrogen and phosphorus, and with spent mushroom substrate. Although both bioaugmentation and biostimulation showed promising results, biostimulation with N, P was the most effective for site-specific bioremediation of Lagoon 3, achieving 67% of oil vegetable reduction at 60 days.
  • Thumbnail Image
    Item
    Site-specific bioremediation by bioaugmentation and biostimulation approaches on a chronically hydrocarbon-contaminated industrial soil.
    (2023-09) Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
    The present study describes practical implication of bioaugmentation and biostimulation processes for bioremediation of an industrial soil chronically contaminated by hydrocarbons. For this purpose, biomass production of six autochthonous hydrocarbon-degrading bacteria were evaluated as inoculum of bioaugmentation strategy, by testing carbon and nitrogen sources included co-products and agro-industrial waste as sustainable and low-cost components of the growth medium. Otherwise, biostimulation was approached by the addition of optimized concentration of nitrogen and phosphorus. Microcosm assays showed that total hydrocarbons (TH) were signifcantly removed from chronically contaminated soil undergoing bioremediation treatment. Systems Mix (bioaugmentation); N,P (biostimulation) and Mix+N,P (bioaugmentation and biostimulation) reached higher TH removal, being 89.85%, 91.00%, 93.04%, respectively, comparing to 77.83% of system C (natural attenuation) at 90 days. The increased heterotrophic aerobic bacteria and hydrocarbon degrading bacteria counts were according to TH biodegrading process during the experiments. Our results showed that biostimulation with nutrients represent a valuable alternative tool to treat a chronically hydrocarbon-contaminated industrial soil, while bioaugmentation with a consortium of hydrocarbon degrading bacteria would be justifed when the soil has a low amount of endogenous degrading microorganisms. Furthermore, the production of inoculum for application in bioaugmentation using low-cost substrates, such as industrial waste, would lead to the development of an environmentally friendly and attractive process in terms of cost–beneft.
  • Thumbnail Image
    Item
    Removal of vegetable oils from contaminated coastal soil by bioaugmentation and biostimulation
    (Congreso Latinoamericano de Ecología Microbiana, 2023-08) Conde Molina, Debora; Corpus, Athina; Piperata, Gabriela
    The environment is negatively impacted by occasional discharges from industrial activity. When these events contain insoluble compounds such as fats and oils, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Zárate-Campana, Buenos Aires, Argentina, where a lagoon was contaminated by vegetable oil residues. The aim of this work is to study bioremediation strategies in order to propose solutions for the remediation of the lagoon. For this purpose, different site-specific treatments were evaluated through microcosms systems using contaminated coastal soil. These treatments were: a control as natural attenuation; a bioaugmentation treatment with autochthonous vegetable oil degrading bacteria (5x1010 CFU/g); two biostimulation treatments with nitrogen (NaNO3, 1 g/Kg) and phosphorus (Na2HPO4, 0.2 g/Kg), and with spent mushroom substrate (10 %w/w). The microcosms carried out with 200 g of soil were incubated at 22 °C for 60 days. Samples were taken every 20 days in order to determine moisture, pH, biological activity by counting total aerobic heterotrophic bacteria and oil degrading bacteria, and total oil concentration through FTIR. The treatments showed oil removal above 43 %, being the most efficient the biostimulation with nitrogen and phosphorus, which reached 58 % degradation after 60 days. Furthermore, the evolution of oil degradation correlated with the increase in microbiological activity in all systems. We concluded that biostimulation with nitrogen and phosphorus was the most appropriate strategy to apply for lagoon remediation.
  • Thumbnail Image
    Item
    Caracterización de residuos agroindustriales con potencial aplicación en procesos biotecnológicos
    (2023-09) Conde Molina, Debora; Di Gregorio, Vanina
    El desarrollo de bioprocesos que utilizan residuos agroindustriales constituye uno de los retos más interesantes de la biotecnología actual, ya que conlleva a la reutilización de residuos de una manera ambientalmente responsable y al desarrollo de productos de alto valor agregado. En este trabajo se caracterizaron residuos agroindustriales de la región noroeste de la provincia de Buenos Aires, con el fin de analizar sus posibles aplicaciones en el desarrollo de bioprocesos locales. Los residuos analizados fueron: compost agotado de hongos, residuo de texturizado de soja, residuo de raíz de batata, residuo de cama de pollo y rastrojo de trigo. A los mismos se les determinó humedad, pH, carbono orgánico, nitrógeno total, fósforo total, potasio, calcio. Los resultados mostraron que todos los residuos contribuyen a cubrir parte de los requerimientos nutricionales de los microorganismos, aportando principalmente fuentes de carbono y nitrógeno.
  • Thumbnail Image
    Item
    Aprovechamiento de residuos de raíz de batata para la producción de xilanasas bacterianas: un enfoque sustentable.
    (UTN Facultad Regional San Francisco, 2023-09) Conde Molina, Debora; Bogao, Guillermina; Tubio, Gisela; Corbino, Graciela
    La producción del cultivo de batatas genera una cantidad significativa de residuos en la región noroeste de la provincia de Buenos Aires, Argentina. Con el fin desarrollar un bioproceso que emplee residuos de raíz de batata para la producción de xilanasas, enzimas de gran aplicación industrial, se aisló y estudió la cepa BX1. El crecimiento de la bacteria y su actividad de xilanasas se evaluó en cultivos líquidos de MSM (medio salino mínimo)+ batata, MSM + xilano y MSM + cáscara de batata. Los resultados mostraron que la cepa BX1 presentó la capacidad de producir xilanasas en un bioproceso diseñado en 2 etapas, primero un batch con medio MSM + batata (5 %p/v) para generar biomasa, y luego esta biomasa se cosechó y empleó en un batch con medio MSM + xilano (0,5 %p/v) para inducir la producción de xilanasas. Esta actividad enzimática se localizó en la fracción extracelular.