Facultad Regional Delta

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Author: search.filters.author.Liporace, FrancoSubject: search.filters.subject.Bioremediation

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    Bioremediation of an industrial soil contaminated by hydrocarbons in microcosm system, involving bioprocesses utilizing co-products and agro-industrial wastes
    (2023-09-29) Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
    The present study describes practical implication of bioaugmentation and biostimulation processes for bioremediation of an industrial soil chronically contaminated by hydrocarbons. For this purpose, biomass production of six autochthonous hydrocarbon-degrading bacteria were evaluated as inoculum of bioaugmentation strategy, by testing carbon and nitrogen sources included co-products and agro-industrial waste as sustainable and low-cost components of the growth medium. Otherwise, biostimulation was approached by the addition of optimized concentration of nitrogen and phosphorus. Microcosm assays showed that total hydrocarbons (TH) were significantly removed from chronically contaminated soil undergoing bioremediation treatment. Systems Mix (bioaugmentation); N,P (biostimulation) and Mix + N,P (bioaugmentation and biostimulation) reached higher TH removal, being 89.85%, 91.00%, 93.04%, respectively, comparing to 77.83% of system C (natural attenuation) at 90 days. The increased heterotrophic aerobic bacteria and hydrocarbon degrading bacteria counts were according to TH biodegrading process during the experiments. Our results showed that biostimulation with nutrients represent a valuable alternative tool to treat a chronically hydrocarbon-contaminated industrial soil, while bioaugmentation with a consortium of hydrocarbon degrading bacteria would be justified when the soil has a low amount of endogenous degrading microorganisms. Furthermore, the production of inoculum for application in bioaugmentation using low-cost substrates, such as industrial waste, would lead to the development of an environmentally friendly and attractive process in terms of cost–benefit.
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    Site-specific bioremediation by bioaugmentation and biostimulation approaches on a chronically hydrocarbon-contaminated industrial soil.
    (2023-09) Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
    The present study describes practical implication of bioaugmentation and biostimulation processes for bioremediation of an industrial soil chronically contaminated by hydrocarbons. For this purpose, biomass production of six autochthonous hydrocarbon-degrading bacteria were evaluated as inoculum of bioaugmentation strategy, by testing carbon and nitrogen sources included co-products and agro-industrial waste as sustainable and low-cost components of the growth medium. Otherwise, biostimulation was approached by the addition of optimized concentration of nitrogen and phosphorus. Microcosm assays showed that total hydrocarbons (TH) were signifcantly removed from chronically contaminated soil undergoing bioremediation treatment. Systems Mix (bioaugmentation); N,P (biostimulation) and Mix+N,P (bioaugmentation and biostimulation) reached higher TH removal, being 89.85%, 91.00%, 93.04%, respectively, comparing to 77.83% of system C (natural attenuation) at 90 days. The increased heterotrophic aerobic bacteria and hydrocarbon degrading bacteria counts were according to TH biodegrading process during the experiments. Our results showed that biostimulation with nutrients represent a valuable alternative tool to treat a chronically hydrocarbon-contaminated industrial soil, while bioaugmentation with a consortium of hydrocarbon degrading bacteria would be justifed when the soil has a low amount of endogenous degrading microorganisms. Furthermore, the production of inoculum for application in bioaugmentation using low-cost substrates, such as industrial waste, would lead to the development of an environmentally friendly and attractive process in terms of cost–beneft.
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    Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
    (2022-04) Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
    These days, petroleum hydrocarbon pollution has become a global problem, because of this, bioremediation is presented as a strategy for cleaning up sites contaminated with organic pollutants, and it has an increasing role in relation to the potential it presents as a non-invasive and cost-effective technology. The aim of this study is to optimize the biomass production of Pseudomonas sp. MT1A3 strain as a soil bioremediation approach for petroleum hydrocarbon polluted environments. Factorial experimental designs were employed to study the effect of several factors of composition medium and incubation conditions on biomass production. Agro-industrial wastes such as peanut oil as carbon source, NaNO3 as nitrogen source and incubation temperature were found to be significant independent variables. These factors were further optimized using Box–Behnken design. Combination of peanut oil 18.69 g/L, NaNO3 2.39 g/L and 26.06 °C incubation temperature was optimum for maximum biomass production of MT1A3 and the model validated in a bioreactor allowed to obtain 9.67g/L. Based on these results, this autochthonous strain was applied in bioaugmentation as a bioremediation strategy through microcosm designs, reaching 93.52% of total hydrocarbon removal at 60 days. This constitutes a promising alternative for hydrocarbon-contaminated soil.
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    Bioremediation strategies based on a native strain isolated from sites contaminated with hydrocarbons.
    (Argentine Society for Biochemistry and Molecular Biology, 2016-11) Conde Molina, Débora; Liporace, Franco; Vázquez, Susana; Merini, Luciano; Quevedo, Carla
    The bacterial strain studied in this work is a member of a bacterial consortium isolated from chronically hydrocarbon-contaminated site in Campana (Bs.As.). This native strain was identified as Pseudomonas sp. according to its 16S rRNA gene partial sequence. The ability of this strain to produce biosurfactants was evaluated in Erlenmeyer flasks containing a minimal saline medium (MSM) supplemented with different carbon sources: a mixture of hydrocarbons (HC; 4.5%v/v), glycerol (Gly; 2%v/v), sunflower oil (SO; 2%v/v) and peanut oil (PO; 2%v/v). Cultures were performed at 135 rpm and 25 ºC for 4 days. Bacterial growth was measured by cell dry-weight method, and biosurfactant production was estimated by direct measurement of the surface tension (ST). Results showed that bacteria was able to grow on all the carbon sources tested, reaching concentrations of 1.24g/l in HC, 7.69g/l in Gly, 3.98g/l in SO and 9.29g/l in PO. Culture supernatants showed a decrease in ST values when the strain grew on SO and PO (22.5% and 25.5%). No decrease in ST values was observed when HC and Gly were used as carbon source. As results shown, this bacterial strain can produce biosurfactants under certain culture conditions. This potential advantage could be applied in bioremediation strategy of hydrocarbon contaminated sites.