Grupo UTN GISTAQ - Difusión Científica - Trabajos Presentados en Congresos

Permanent URI for this collectionhttp://48.217.138.120/handle/20.500.12272/3709

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    Formación de biopelículas para el tratamiento de efluentes contaminados con furfural
    (2023-08-29) Echeverría, Macarena Celeste; Sandoval, Evangelina; Gauto, Víctor Hugo; Benimeli, Claudia Susana
    El furfural es un aldehído heterocíclico, con variadas aplicaciones en la industria química. A pesar de los beneficios que posee su uso y producción, el aldehído se convierte en un componente tóxico importante en las aguas residuales si es liberado al medio ambiente sin un tratamiento adecuado (Lei y col., 2022; Rashtbari y col., 2022). Los cultivos inmovilizados en biopelículas representan una herramienta biológica eficiente para la eliminación de contaminantes en los procesos de biorremediación (Isaac y col., 2017). En el presente trabajo se evaluó la capacidad de formación de biopelículas por las cepas de actinobacterias Nocardiopsis sp. L9 y Streptomyces sp. A12 y M7, en cultivos mixtos y puros, sobre esponja vegetal, y la eficiencia de dichas biopelículas para la remoción de furfural en un efluente simulado. Como resultado se obtuvieron diferencias significativas (p<0,05) en la actividad enzimática hidrólisis de FDA entre el soporte de esponja vegetal estéril (sin colonizar) y los tratamientos con esponja colonizada por las cepas puras y en consorcio, indicando la actividad biológica del biofilm. El análisis por microscopía electrónica de barrido confirmó un elevado desarrollo bacteriano adherido a la superficie de la esponja vegetal con presencia de posibles sustancias poliméricas extracelulares. Durante el proceso de biorremediación del efluente simulado contaminado con furfural (831±23 mg l-1), se observaron concentraciones residuales nulas al tercer día de tratamiento, al utilizar el biofilm formado por el cultivo mixto de las actinobacterias Nocardiopsis sp. L9 y Streptomyces sp. A12 y M7. Los resultados obtenidos sugieren que dichas biopelículas representan una herramienta de biorremediación prometedora para el tratamiento de efluentes que contienen furfural.
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    Furfural removal from a polluted effluent by using a fluidized bed reactor
    (2023-10-02) Echeverría, Macarena Celeste; Cuadra, Pablo Nicolás; Sandoval, Evangelina; Benimeli, Claudia Susana
    Furfural is a heterocyclic aromatic aldehyde and wastewaters derived from its production can contain around 800 mg l-1, which can cause toxic effects on living systems if released into the environment without proper treatment. In the present work, the furfural removal from a simulated effluent by a fluidized bed bioreactor filled with an actinobacteria biofilm on vegetable sponge (Luffa aegyptiaca) support was studied. For this, a suspension of a mixed culture of Nocardiopsis sp. L9, Streptomyces sp. A12 and M7, in TSB medium (D.O540nm=1) was prepared. The luffa support was cut in cubes of approximately 0.1 g, which were washed and sterilized. The bacterial biofilm production on luffa cubes was carried out in 250 ml Erlenmeyer flasks, which contained 0.5 g of the support and 60 ml of the bacterial suspension. After 96 h of incubation at 30 °C and 100 rpm, the colonized sponge cubes were introduced into the reactor for the bioremediation treatment. A laboratory-scale fluidized bed reactor was used, which had an inlet for the effluent to be treated in the lower side and an outlet for the treated effluent in the upper part. The furfural residual concentration in the treated effluent was evaluated by HPLC, every 24 h for 4 days. Ecotoxicity tests were carried out using Raphanus sativus seeds (radish, Punta Blanca variety). Bacterial colonization on vegetal sponge was also evaluated by scanning electron microscopy, before and after treatment. The analysis by HPLC showed a complete depletion of furfural in the effluent after 24 h of treatment. Microphotographs by scanning electron microscopy showed an increase in the presence of possible polymeric substances in luffa cubes at the end of treatment regarding to the initial time, as result of biofilm production by the actinobacterial consortium. The ecotoxicity tests with radish seeds showed significant increases (p<0.05) in the vegetable biomarkers of seedlings obtained in the treated effluent, indicating that the toxic effects caused by furfural were reversed, confirming the effectiveness of the bioremediation process.