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    Potential of vegetable oil degrading bacteria as inoculum for bioaugmentation in the remediation of contaminated sites.
    (Encuentro Internacional de Ciencias de la Tierra, 2022-11) Conde Molina, Debora; Sanchez Hulmedilla, Celene; Silva, Fausto; Piperata, Gabriela
    The environment is negatively impacted by occasional discharges from industrial activity. When these discharges contain insoluble compounds such as fats, oils and grease, they are high impact pollutants. This work focuses on an environmental problem in the industrial area of Campana, Buenos Aires, where a vegetable oil residues treatment industry generated a significant uncontrolled discharge of waste into Lagoon 3 of the private nature reserve El Morejón. In view of this, there is a need to address bioremediation strategies to clean up the area. The aim of this work is to study growth conditions of vegetable oil degrading bacterial consortia, previously isolated from lagoon 3, with a view to applying it as an inoculum in bioaugmentation strategy. For this purpose, bacteria were tested in flask containing liquid culture media at different conditions, such as: medium formulated with 2-5 % v/v vegetable oil -as only carbon source-, shaking at 135-220 rpm, medium formulated with alternative carbon source of sweet potato waste (5% w/v). The best condition for the growth of bacteria was a culture medium formulated with 5 % v/v vegetable oil, incubated at 135 rpm for days, reaching 9 g/L of biomass. Moreover, this condition maintains the selection pressure so that bacteria preserve the ability to degrade vegetable oils. The significant biomass obtained positions these autochthonous bacteria with great potential to be applied as bioaugmentation in site-specific bioremediation strategy for the remediation of Lagoon 3.
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    Optimization of biomass production by autochthonous Pseudomonas sp. MT1A3 as a strategy to apply bioremediation in situ in a chronically hydrocarbon-contaminated soil.
    (2022-04) Conde Molina, Debora; Liporace, Franco; Quevedo, Carla
    These days, petroleum hydrocarbon pollution has become a global problem, because of this, bioremediation is presented as a strategy for cleaning up sites contaminated with organic pollutants, and it has an increasing role in relation to the potential it presents as a non-invasive and cost-effective technology. The aim of this study is to optimize the biomass production of Pseudomonas sp. MT1A3 strain as a soil bioremediation approach for petroleum hydrocarbon polluted environments. Factorial experimental designs were employed to study the effect of several factors of composition medium and incubation conditions on biomass production. Agro-industrial wastes such as peanut oil as carbon source, NaNO3 as nitrogen source and incubation temperature were found to be significant independent variables. These factors were further optimized using Box–Behnken design. Combination of peanut oil 18.69 g/L, NaNO3 2.39 g/L and 26.06 °C incubation temperature was optimum for maximum biomass production of MT1A3 and the model validated in a bioreactor allowed to obtain 9.67g/L. Based on these results, this autochthonous strain was applied in bioaugmentation as a bioremediation strategy through microcosm designs, reaching 93.52% of total hydrocarbon removal at 60 days. This constitutes a promising alternative for hydrocarbon-contaminated soil.
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    Use of sweet potato root residues as carbon source for the growth of xylanase-producing Cellulosimicrobium sp.
    (NUFT, 2021-09) Conde Molina, Débora; Corbino, Graciela
    Application of sweet potato root (SRP) residues as an alternative carbon source to formulate a culture medium for the growth of xylanase-producing microorganisms, is presented in this article. Six commercial varieties of SPR were tested for evaluating the growth of Cellulosimicrobium sp. CO1A strain in shaken flask cultures. Results showed that Cellulosimicrobium sp. CO1A1 was able to grow in a medium formulated with SPR. Biomass estimated by optical density was the most appropriate method for the medium evaluated. Additionally, biomass did not show significant differences (p>0.05) between commercial varieties of SPR, being between 8.12 and 9.26 of OD. All the varieties of RPS tested in this work are equally promising source to be applied in a bioprocess to obtain xylanase by Cellulosimicrobium sp. CO1A1.