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Author: search.filters.author.Liebana, ClaraStart date: 2010

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    Antioxidant and functional properties of bioactive peptide fractions derived from shrimp (Pleoticus muelleri) waste hydrolysates.
    (Elsevier, 2025-06-16) Liebana, Clara; Pereira, Nair de los Angeles; Fernandez-Gimenez, Analía Veronica; Granone, Luis Ignacio; Fangio, María Florencia
    Protein hydrolysates were prepared from shrimp (Pleoticus muelleri) waste through autolysis (Aut) and using 1 % and 2 % (v/v) Alcalase (Alc 1 and Alc 2, respectively). The degree of hydrolysis (DH) and antioxidant activities were assessed via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+) scavenging assays at various hydrolysis times. After 90 min, Aut and Alc 1 were selected for fractionation by ultrafiltration, yielding peptide fractions: F1 (>10 kDa), F2 (3–10 kDa), and F3 (<3 kDa). Functional properties, antioxidant capacities, and lipid peroxidation inhibition in raw fish emulsions were assessed for both whole hydrolysates (WH) and their peptide fractions. Alc 1 achieved a significantly higher DH (42.02 ± 5.50 %) than Aut (28.75 ± 2.34 %). Both hydrolysates and their fractions displayed high solubility (>65.49 ± 2.07 %) across a wide pH range. The F1 fraction exhibited the strongest DPPH scavenging activity for both hydrolysates, with Aut's F1 reaching 445.90 ± 19.52 mg AEAC/100 g dry weight and Alc 1's F1 achieving 461.10 ± 15.81 mg AEAC/100 g dry weight. Aut's F3 fraction had the highest ABTS•+ scavenging capacity (723.66 ± 36.58 mg AEAC/100 g dry weight), while Alc 1's WH exhibited superior activity (776.20 ± 52.55 mg AEAC/100 g dry weight). Overall, Aut's F1 fraction demonstrated the most favorable antioxidant and functional properties, while Alc 1's F3 fraction showed superior solubility and lipid peroxidation inhibition. These findings underscore the potential of shrimp (P. muelleri) waste for producing functional hydrolysates and bioactive peptides with diverse applications.
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    Converting agricultural and fisheries waste into high-value products: Utilization of pea meal and shrimp protein hydrolysate as feed components for Nile Tilapia (Oreochromis niloticus)
    (Iranian Society of Ichthyology, 2025-02-25) Liebana, Clara; Rodriguez, Yamila Eliana; Fernández-Gimenez, Analía Verónica; Cecchi, Federico Alcides; Pereira, Nair de los Ángeles
    This study aims to characterize pea meal and shrimp protein hydrolysate as alternative protein ingredients, replacing soybean meal (SBM) in tilapia feed. The presence of protease inhibitors and the in vitro digestion were evaluated for both raw and extruded pea meals, while the characteristics of shrimp protein hydrolysate were also studied. The nutritional quality of alternative ingredients was assessed to formulate a diet that meets tilapia juveniles’ requirements, and its preliminary effects on growth were evaluated. Extrusion significantly reduced the inhibitory effect of pea meal on tilapia proteases (from 5.4 to 1.9%). In vitro, protein digestion of extruded pea meal (7.8±1.35%) did not differ from the control treatment with fish meal (11.8±1.94%). Additionally, shrimp protein hydrolysate presented 41.6±2.91% of DPPH radical scavenging activity. Tilapia fed a diet including 25% extruded pea meal and 10% shrimp protein hydrolysate, replacing soybean meal, presented comparable growth indicators to those exposed to the control diet. All the results in this study demonstrate that it is possible to include these ingredients derived from industrial waste in tilapia diets, improving the quality of the feed without affecting its performance.
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    From waste to value: protein hydrolysates from byproducts of the Argentine hake (Merluccius hubbsi) processing using endogenous enzymes and Alcalase® 2.4L = De residuo a valor: hidrolizados proteicos de subproductos del procesamiento de la merluza argentina (Merluccius hubbsi) utilizando enzimas endógenas y Alcalasa® 2.4L.
    (INIDEP, 2025-03-19) Liebana, Clara; Pereira, Nair de los Ángeles; Fernández-Gimenez, Analia V.; Fangio, María Florencia
    La valorización de los subproductos pesqueros es fundamental para reducir los residuos y crear productos de alto valor. Los residuos de la merluza argentina (Merluccius hubbsi) podrían potenciar sus propiedades funcionales y antioxidantes a través de la hidrólisis, liberando péptidos con propiedades bioactivas. Los hidrolizados proteicos de merluza argentina tienen un gran potencial como ingredientes funcionales debido a sus propiedades bioactivas, pero optimizar los procesos de hidrólisis es esencial para mejorar el rendimiento y las características biofuncionales, como la actividad antioxidante. Se obtuvieron hidrolizados proteicos de merluza argentina mediante autólisis (Aut) y hidrólisis enzimática utilizando AlcalasaÒ 2.4 L a concentraciones de 0,24% y 2% (v/v) (Alc-0.24 y Alc-2), respectivamente, durante 150 min. Se evaluó la actividad de peptidasas alcalinas, el grado de hidrólisis y la actividad antioxidante utilizando ensayos de inhibición del radical 2,2-difenil-1-picrilhidrazilo (DPPH) y 2,2’-azino-bis (ácido 3-etilbenzotiazolina-6-sulfónico) ABTS·+. Todos los hidrolizados mantuvieron actividad de peptidasas alcalinas a lo largo del proceso. Los hidrolizados tratados con Alcalasa® 2.4L mostraron una actividad de peptidasas e un grado de hidrólisis significativamente mayores en comparación con Aut. A los 60 min, Alc-0.24 alcanzó niveles de actividad de peptidasas similares a los de Alc-2, y a los 30 min, ambos presentaron grados de hidrólisis comparables. La actividad de captura de ABTS·+ aumentó con el tiempo para Alc-0.24, siendo ambas concentraciones de Alcalasa® 2.4 L superiores a la autólisis. No se encontraron diferencias significativas entre Alc-0.24 y Alc-2. Aunque todos los hidrolizados mostraron actividad de captura de DPPH, no se detectaron diferencias significativas entre tratamientos o tiempos de reacción. Estos hallazgos destacan el potencial para producir hidrolizados proteicos de valor agregado a partir de residuos de merluza argentina.